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Coding
Part:BBa_K3595006:Design
Designed by: Ying Li Group: iGEM20_GZ_HFI (2020-10-26)
Mutant-cysE-5
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 684
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Substituting GTC (Val), GAT (Asp) at the 95th and 96th positions of wild-type cysE sequence for AGA (Arg), CCC (Pro)
Source
synthetic