Coding

Part:BBa_K3595006:Design

Designed by: Ying Li   Group: iGEM20_GZ_HFI   (2020-10-26)


Mutant-cysE-5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Substituting GTC (Val), GAT (Asp) at the 95th and 96th positions of wild-type cysE sequence for AGA (Arg), CCC (Pro)


Source

synthetic

References